Table 2

pIC50 values (M) for peptides in competition for binding of125I-rat amylin to CHO-P cells transfected with hCTRl1+(100 ng) and RAMP or vector DNA (150 ng)

sCTSiteshCTSitesRat AmylinSiteshCGRPαSites
% % % %
pcDNA3
 Site 111.06  ± .01556.9  ± 3.810.93  ± 0.3242.3  ± 5.410.02  ± 0.3238.0  ± 4.911.08  ± 0.426.8  ± 3.6
 Site 28.12  ± 0.1943.16.78  ± 0.2557.75.65  ± 0.25625.77  ± 0.1773.2
RAMP1
 Site 111.2  ± 0.11 2-15087.6  ± 3.39.89  ± 0.3356  ± 9.8 2-15011.29  ± 0.1944.4  ± 5.011.89  ± 0.5623.2  ± 6.3
 Site 26.41  ± 0.812.46.95  ± 0.4244 2-1608.66  ± 0.1655.6 2-1598.64  ± 0.1876.8
RAMP2
 Site 111.83  ± 0.2864.0  ± 9.49.63  ± 0.2859.5  ± 7.710.65  ± 0.3836.9  ± 7.010.96  ± 0.4520.6  ± 4.5
 Site 28.83  ± 0.51366.42  ± 0.4140.5 2-1657.59  ± 0.2363.1 2-1657.71  ± 0.1279.4
RAMP3
 Site 111.53  ± 0.2169.7  ± 8.410.19  ± 0.3767.9  ± 9.7 2-15011.55  ± 0.3647.4  ± 7.610.1  ± 0.35 2-16758.8  ± 10.1
 Site 28.98  ± 0.4530.35.95  ± 0.8332.1 2-1508.2  ± 0.3152.6 2-2156.9  ± 0.5141.2

Data are presented as mean ± S.E. (n = 3).

  • 2-150 P < .05 versus pcDNA3;

  • 2-160 P < .05 versus pcDNA3, R2;

  • 2-165 P < .05 versus pcDNA3, R1;

  • 2-159 P < .05 versus pcDNA3, R2, R3;

  • 2-167 P < .05 versus pcDNA3, R2, R1;

  • 2-215 P < .05 versus R1.