Effect of dose and intrahepatic concentrations of MC on hepatic EROD and MROD activities
| Dose of MC | Intrahepatic Concentration of MC | CYP Contents | EROD Activities | MROD Activities |
|---|---|---|---|---|
| μmol/kg | pmol/g | nmol/mg of protein | nmol/min/nmol of P450 | |
| 0 | 0.66 ± 0.09 | 0.41 ± 0.03 | 0.91 ± 0.09 | |
| 0.1 | 1.3 ± 0.2 | 0.70 ± 0.06 | 0.48 ± 0.09 | 0.76 ± 0.23 |
| 0.5 | 33.5 ± 13.5 | 0.75 ± 0.05 | 0.53 ± 0.07 | 1.05 ± 0.88 |
| 1.0 | 93.0 ± 13.0 | 0.78 ± 0.08 | 0.56 ± 0.09 | 0.88 ± 0.04 |
| 2.0 | 271.0 ± 29.0 | 0.76 ± 0.07 | 0.62 ± 0.15 | 1.08 ± 0.16 |
| 5.0 | 525.0 ± 54.6 | 1.00 ± 0.13 | 3.67 ± 0.78 | 2.80 ± 0.66 |
| 10.0 | 826.0 ± 83.7 | 1.12 ± 0.15 | 8.61 ± 1.10 | 5.80 ± 1.53 |
| 25.0 | 1882.0 ± 182.5 | 1.36 ± 0.17 | 11.52 ± 1.80 | 8.80 ± 1.30 |
| 50.0 | 1850.0 ± 70.2 | 1.46 ± 0.19 | 13.20 ± 0.64 | 8.60 ± 0.81 |
| 100.0 | 1934.0 ± 93.3 | 1.49 ± 0.19 | 14.50 ± 1.88 | 9.16 ± 1.64 |
CYP contents and EROD and MROD activities represent mean values of liver samples from at least three individual animals. Values depicting intrahepatic concentrations represent means of data from three individual animals. The intrahepatic concentrations of MC in animals given [3H]MC were determined as given in Materials and Methods.