Checkerboard analysis of anandamide-induced migration of 293 cells stably transfected with human CB1 gene
| Anandamide in Lower Chamber (M) | Anandamide in Upper Chamber (M) | |||
|---|---|---|---|---|
| 0 | 3 × 10−8 | 1 × 10−7 | 3 × 10−7 | |
| 0 | 1.12 ± 0.13 | 1.23 ± 0.11 | 1.33 ± 0.11 | 1.13 ± 0.15 |
| 3 × 10−8 | 2.33 ± 0.34 | 1.99 ± 0.26 | 2.27 ± 0.36 | 2.16 ± 0.47 |
| 1 × 10−7 | 4.69 ± 0.65 | 4.05 ± 0.52 | 3.82 ± 0.53 | 3.85 ± 0.66 |
| 3 × 107 | 7.79 ± 1.69 | 5.42 ± 1.07 | 5.51 ± 0.99 | 5.54 ± 1.04 |
Results are expressed as migration index as described inExperimental Procedures. Data represent mean ± S.E. of three separate assays, each conducted in triplicate.