Effect of bilobalide on c-Myc, p53, Bcl-xL, and Bax protein expression in PC12 cells treated with xanthine (100 μM)/xanthine oxidase (150 mU/ml)
| Control | Xanthine/Xanthine Oxidase + Bilobalide | ||||
|---|---|---|---|---|---|
| 0 | 12.5 | 25 | 50 | ||
| μm | |||||
| c-Myc (3 h) | 8.4 ± 6.9 | 27.8 ± 3.6 | 24.6 ± 6.2 | 16.7 ± 3.81-150 | 11.3 ± 5.41-150 |
| p53 (3 h) | 6 ± 3.4 | 50.1 ± 12.4 | 42.8 ± 7.3 | 23.2 ± 12.71-150 | 14.6 ± 4.9** |
| Bax (12 h) | 45.9 ± 7.6 | 89.8 ± 7.1 | 88.5 ± 6.1 | 76.4 ± 9.5 | 66.2 ± 10.71-150 |
| Bcl-xL (12 h) | 3 ± 1.2 | 3.4 ± 1.7 | 4.75 ± 1.2 | 3.9 ± 2.6 | 2.6 ± 1.4 |
The percentage of cells with positive staining was determined with flow cytometric assay. The levels of c-Myc and p53 were observed after 3 h of treatment with xanthine/xanthine oxidase. The levels of Bcl-xL and Bax were measured after 12 h of treatment with xanthine/xanthine oxidase. Data are mean ± S.E. of three independent experiments.
↵1-150 P < .05, ** P < .01 versus group treated with xanthine/xanthine oxidase alone.