Comparative analysis of BBB permeability after NO prodrug infusion
| NO Prodrug | Average [14C]Sucrose Clin Ipsilateral Forebrain | Average [14C]Sucrose Clin | Predicted NO Congener Release in the Cerebrovasculature | ΔMAP at 60 min |
|---|---|---|---|---|
| % osmotic opening | μM | % change from baseline | ||
| Thionitrite (NO·, NO−, NO+) | ||||
| Saline | 4.61 ± 0.54 | 0.00 | 0 | |
| CD | 4.28 ± 0.60 | 0.00 | 0 | −2.17 ± 2.44 |
| SNAPcbv (0.191 μmol/min) | 5.18 ± 0.64 | 1.00 | 9.7 | −9.88 ± 5.28 |
| SNAP-CDfem(1.7 mmol/min) | 5.31 ± 0.24 | 1.20 | 0.016 | −31.6 ± 9.983-150 |
| SNAP-CDcbv (1.7 mmol/min) | 30.4 ± 1.473-151 | 44.24 | 86 | −31.2 ± 1.113-150 |
| Nitroxyl agents (NO·, NO−) | ||||
| Angeli's Salt | 14.9 ± 0.65*** | 17.65 | 47 | −25.4 ± 10.83-150 |
| Piloty's Acid | 5.28 ± 0.67 | 1.15 | N.D. | −1.15 ± 8.14 |
| Diazeniumdiolates (NO·) (i.e. NONOates) | ||||
| Saline | 6.32 ± 0.76 | 0.00 | 0 | 4.73 ± 10.4 |
| MAHMA NONOate | 11.0 ± 0.37** | 8.27 | 415 | −42.2 ± 11.8** |
| Diethylamine NONOate | 11.2 ± 0.94** | 8.62 | 205 | −51.2 ± 4.52** |
| Spermine NONOate | 8.88 ± 0.393-150 | 4.52 | 12 | −53.9 ± 3.24** |
| DETA NONOate | 6.39 ± 0.74 | 0.12 | 0.37 | −24.3 ± 15.3 |
Data are mean ± S.E. of three to six rats. The drug infusions were: each nitroxyl agent, 1.7 mmol/min; each NONOate agent, 1.7 mmol/min. Statistical significance was assessed by one-way ANOVA with Newman-Keuls post hoc test. Data with dissimilar (or no) superscripts are statistically different from other drug treatments and are statistically similar to values with the same superscript. The %osmotic opening (relative to mannitol) was calculated as (test drug Clin − mean control Clin)/(mean mannitol Clin− mean control Clin) · 100%.