Ipsilateral forebrain regional [14C] sucrose Clinafter cerebrovascular NO prodrug administration
| NO Prodrug | Frontal Cortex | Parietal Cortex | Hippocampus | Thalamus/Hypothalamus | Striatum |
|---|---|---|---|---|---|
| Control | |||||
| No drug | 7.31 ± 1.08 | 6.42 ± 0.57 | 4.91 ± 0.73 | 6.65 ± 1.04 | 5.81 ± 0.86 |
| Mannitol | 71.8 ± 12.91-151 | 57.2 ± 10.21-151 | 51.6 ± 6.821-151 | 56.2 ± 8.081-151 | 63.8 ± 11.01-151 |
| Thionitrite (NO·, NO−, NO+) | |||||
| Saline | 4.41 ± 1.46 | 4.43 ± 1.54 | 5.12 ± 1.66 | 3.98 ± 1.62 | 4.91 ± 0.90 |
| SNAPcbv (0.19 μmol/min) | 5.77 ± 1.73 | 4.60 ± 1.19 | 4.40 ± 2.18 | 4.86 ± 1.96 | 4.85 ± 1.22 |
| CD | 4.64 ± 1.51 | 4.34 ± 1.61 | 2.66 ± 1.94 | 4.69 ± 1.36 | 4.28 ± 1.33 |
| SNAP-CDfem (1.7 mmol/min) | 6.00 ± 0.62 | 5.08 ± 0.56 | 5.75 ± 0.62 | 5.22 ± 0.72 | 4.13 ± 0.56 |
| SNAP-CDcbv(1.7 mmol/min) | 27.2 ± 2.001-151 | 26.7 ± 3.421-151 | 30.3 ± 5.121-151 | 32.8 ± 4.471-151 | 31.4 ± 2.861-151 |
| Nitroxyl agents (NO·, NO−) | |||||
| Saline1-a | 5.58 ± 0.18 | 5.15 ± 0.21 | 8.47 ± 2.22 | 6.39 ± 2.00 | 6.28 ± 0.12 |
| Piloty's Acid | 5.51 ± 0.16 | 4.94 ± 0.15 | 5.09 ± 0.17 | 6.04 ± 2.24 | 4.53 ± 0.19 |
| Angeli's Salt | 14.3 ± 0.23*** | 11.4 ± 0.02** | 16.6 ± 0.11*** | 16.2 ± 1.46*** | 13.5 ± 0.09*** |
| Diazeniumdiolates (NO·) (i.e. NONOates) | |||||
| MAHMA NONOate | 11.8 ± 0.10** | 11.1 ± 0.08** | 10.4 ± 1.08** | 10.9 ± 0.81** | 10.3 ± 0.08** |
| Diethylamine NONOate | 10.5 ± 0.26** | 12.1 ± 0.25** | 11.6 ± 3.90** | 11.4 ± 1.83** | 11.3 ± 0.22** |
| Spermine NONOate | 8.61 ± 0.221-150 | 7.81 ± 0.091-150 | 9.09 ± 0.651-150 | 9.99 ± 0.941-150 | 7.93 ± 0.121-150 |
| DETA NONOate | 6.97 ± 0.17 | 6.18 ± 0.17 | 6.88 ± 2.28 | 7.05 ± 2.26 | 4.22 ± 0.17 |
Data are mean ± S.E. (n = 5–11 rats) and are corrected for intravascularly entrapped isotope. Clin units: cm3/g of wet wt/min × 10−3. Statistical significance was assessed by two-way ANOVA with Newman-Keuls post hoc test. Data with dissimilar (or no) superscripts are statistically different from other drug treatments and are statistically similar to values with the same superscript. The infusion rates for the SNAP formulation are indicated, and the infusion rate for each remaining NO prodrug was 1.7 mmol/min.
cbv, cerebrovascular administration via the external carotid artery; Clin, influx clearance; fem, systemic administration via the femoral artery.
↵1-a The saline vehicle used for the NONOates and nitroxyl agents contained 10 mM NaOH and was purged with argon gas.
↵1-150 P < .05; **P < .01; ***P < .005;
↵1-151 P < .001.