Effect of ALDH-1 AS expression on enzyme activity and sensitivity to 4-HC of A549 cells
| WT | AS-N | AS-C | AS-NC | |
|---|---|---|---|---|
| Enzyme activity | ||||
| nmol/107cells/min | 760 ± 426-a | 408 ± 76-c | 464 ± 286-c | 202 ± 126-c |
| 4-HC treatment | ||||
| 40 μg/ml | 126 ± 8 (1.5)6-b | 15 ± 5 (0.4)6-c | 7 ± 2 (0.16)6-c | 2 ± 2 (0.03)6-c |
| 60 μg/ml | 18 ± 4 (0.21) | 0 | 0 | 0 |
↵6-a Results represent mean ± S.D. of 10 enzyme activity measurements done at two different time points of cell culture.
↵6-b Results represent mean ± S.D. of total colonies scored in four replicate culture plates, each plated at 2.5 × 104 treated cells/plate. Values in parentheses represent percentage recovery of colonies from 4-HC-treated cells in relation to the number of colonies obtained from untreated cells.
↵6-c Significantly different compared with WT group,P < .005.