Amitriptyline binding to human liver microsomes and microsomes from human B-lymphoblastoid cells or insect cells
| Matrix | Protein Concentration | Free Fraction |
|---|---|---|
| μg/ml | ||
| Pooled human liver microsomes | 50 | 1.075 |
| 100 | 0.878 | |
| 200 | 0.629 | |
| 500 | 0.324 | |
| Pooled human liver microsomes (heat-inactivated) | 200 | 0.502 |
| Lymphoblast microsomes | 250 | 0.816 |
| 500 | 0.55 | |
| 1000 | 0.257 | |
| Insect cell microsomes | 250 | 0.675 |
| Buffer (control) | – | 1.192 |
Values are unbound fractions determined at a spiked drug concentration of 50 μg/ml.