Effect of VMAT2 inhibition during acute MPTP treatment
| VMAT2 Inhibitor | MPTP | DA Content | TH Activity |
|---|---|---|---|
| mg/kg | % control | ||
| Experiment 11-a | |||
| Vehicle (3) | Saline | 100 ± 12 | 100 ± 3 |
| Reserpine (6) | Saline | 71 ± 21-d | 100 ± 4 |
| Vehicle (3) | 30 | 45 ± 111-d | 72 ± 10 |
| Reserpine (3) | 30 | 34 ± 51-d | 75 ± 31-d |
| Experiment 21-b | |||
| Vehicle (3) | Saline | 100 ± 9 | 100 ± 11 |
| Reserpine (3) | Saline | 44 ± 111-d | 127 ± 19 |
| Vehicle (3) | 40 | 49 ± 101-d | 79 ± 10 |
| Reserpine (3) | 40 | 10 ± 31-d 1-e | 95 ± 13 |
| Experiment 31-c | |||
| Vehicle (3) | Saline | 100 ± 4 | N.T.1-f |
| Ro 4-1284 (3) | Saline | 106 ± 2 | N.T. |
| Vehicle (4) | 80 | 24 ± 41-d | N.T. |
| Ro 4-1284 (5) | 80 | 26 ± 21-d | N.T. |
Mice were treated as described in the footnotes. Results are presented as percentage of control ± S.E. for the number of mice indicated in parentheses. DA values (micrograms per gram of tissue) for controls ranged from 10.0 ± 0.5 to 12.8 ± 1.1 (mean ± S.E.); TH values (nanomoles per gram per hour) for controls ranged from 230 ± 21 to 421 ± 10 (mean ± S.E.).
↵1-a Reserpine (2.5 mg/kg) was administered 24 h before MPTP (total dose of 30 mg/kg given in two i.p. injections of 15 mg/kg/injection at 2-h intervals). Mice were sacrificed 28 days later.
↵1-b Reserpine (2.5 mg/kg) was administered 24 h before MPTP (total dose of 40 mg/kg given in three i.p. injections of 10, 10, and 20 mg/kg/injection. Mice were sacrificed 7 days later.
↵1-c Ro 4-1284 (10 mg/kg/injection × three injections at 3-h intervals) and MPTP (20 mg/kg/injection × four injections) were administered concurrently. Mice were sacrificed 7 days later.
↵1-d P < .05 versus vehicle/saline controls.
↵1-e P < .05 versus reserpine group.
↵1-f N.T., not tested.