Comparison of the kinetics for the uptake of [3H]DA and [3H]MPP+ into vesicles isolated from striata of mice and rats
| Mice | Rats | |
|---|---|---|
| [3H]DA uptake | ||
| K m (nM) | 339 ± 35 | 462 ± 68 |
| V max (pmol/mg protein/min) | 69 ± 2 | 156 ± 251-150 |
| [3H]MPP+ uptake | ||
| K m(nM) | 2012 ± 610 | 2767 ± 586 |
| V max(pmol/mg protein/min) | 23 ± 5 | 57 ± 141-150 |
| [3H]DTBZ | ||
| K d (nM) | 1.4 ± 0.3 | 1.5 ± 0.2 |
| B max (pmol/mg protein) | 5.7 ± 0.3 | 11.4 ± 1.61-150 |
| DA turnover (min−1) | 12.2 ± 0.8 | 13.6 ± 1.3 |
| MPP+turnover (min−1) | 3.8 ± 0.3 | 3.9 ± 0.9 |
Kinetic analyses were performed as described in Materials and Methods. Results are the mean ± S.E. of three experiments.
↵1-150 P < .05 compared with respective values in mice (Student's t test).