Competition studies using 125I-angiotensinogen 3–11(Lys11) in guinea pig lung membranes
| Competitors | Ki1 | Ki2 |
|---|---|---|
| Angiotensinogen 3–11(Lys11) | 7.00 ± 1.17 nM (59.6 ± 1.1%) | 0.22 ± 0.08 nM (40.4 ± 1.1%) |
| Bradykinin | 1.10 ± 0.15 × 10−7 M | |
| Angiotensin I | > 3.16 × 10−5M |
Concentration of competitors ranged from 3.16 × 10−5 to 1 × 10−11 M and were constructed by half-log dilutions. Angiotensinogen 3–11(Lys11) best fit a two-site binding model (r2 = 1.000 ± 0.0001; P < .0005). The percentage of the total bound receptor is recorded next to each site. The below data are the mean ± S.E. (n= 3).