Kd | Bmax | |
---|---|---|
nM | fmol/mg protein | |
Control | 1.35 ± 0.14 | 160 ± 30 |
Eto 30 min | 1.18 ± 0.15 | 64.5 ± 101-150 |
Eto 4 h | 0.81 ± 0.25 | 48 ± 8.51-150 |
Control + OA | 1.41 ± 0.12 | 170 ± 25 |
Eto 30 min Res | 1.22 ± 0.12 | 125 ± 17 |
Eto 30 min Res + OA | 0.96 ± 0.34 | 75.5 ± 121-150 |
Eto 4 h Res | 1.14 ± 0.31 | 79 ± 311-150 |
Eto 4 h Res + OA | 0.98 ± 0.18 | 62 ± 71-150 |
The binding of [3H]diprenorphine was determined as described in Materials and Methods in naive cells (control), in cells pretreated with 100 nM Eto during 30 min or 4 h (Eto 30 min and Eto 4 h), and in cells allowed to resensitize 1 h in fresh culture medium (Res) and in the absence or presence of 10 nM OA. Data are mean ± S.D. of three independent experiments.
↵1-150 P < .05, significantly different from control values (t test).