Concentration-dependent increase of recoverable stimulated endogenous ACh from rat primary septal cultures by the AChE inhibitor neostigmine on DIV 8
| Neostigmine | Recovered ACh |
|---|---|
| μM | fmol/well/10 min ± S.E. |
| 0 | 1732 ± 107 |
| 0.001 | 2431 ± 557 |
| 0.1 | 4225 ± 1552-150 |
| 10 | 4642 ± 532-150 |
The values represent the ACh concentration after 10 min of stimulation (K+ = 25 mM) expressed as femtomoles ACh/well/10 min (mean ± S.E.; n = 4). The indicated concentration of neostigmine was also present during the release period as well as a 60-min equilibration period. The two higher doses of neostigmine significantly increased the recovered ACh compared with that in the absence of neostigmine (one-way ANOVA with Tukey's multiple comparison test).
↵2-150 P < .01.