Effect of alprogen on mediator releases from passively sensitized (anti-OA) lung mast cell activation caused by 0.1 μg/ml OA
| Treatment | Histamine | Leukotrienes |
|---|---|---|
| % | pmol/4 × 105cells | |
| OA alone | 29.0 ± 1.10 | 42.9 ± 5.40 |
| Alprogen (μg) | ||
| 0.5 | 23.5 ± 1.211-150 | 40.5 ± 7.53 |
| 1.0 | 18.6 ± 1.90** | 29.8 ± 5.62** |
| 2.5 | 11.1 ± 6.12*** | 25.2 ± 7.85** |
| 5.0 | 7.5 ± 7.50*** | 23.2 ± 6.23*** |
Guinea pig lung mast cells were isolated and purified by digestion and rough and continuous Percoll density gradient method. Mast cells (4 × 105 cells) were passively sensitized by anti-OA antibody and challenged by 0.1 μg/ml OA.
↵1-150 p < .05; ** p < .01; *** p < .001 compared with OA alone.