Binding parameters of CB1 wild type, CB1 A198M, and CB1 G195S to [3H]WIN 55,212-2
| Receptor | Kd | Bmax |
|---|---|---|
| nM | fmol/mg protein | |
| CB1 wild type | 20.4 ± 3.6 | 1926 ± 149 |
| CB1 A198M | 20.4 ± 5.1 | 1949 ± 185 |
| CB1 G195S | 5.6 ± 0.6 | 1534 ± 56 |
Results are obtained from membrane preparations of 293 cells expressing the wild-type and mutant CB1 receptors, and data are presented as mean ± S.E. values from at least four saturation binding experiments performed in duplicate.