Ligand | Net Specific Binding1-afmol/mg | n |
---|---|---|
[3H]MK-801, 40 nM | 143 ± 17.8 | 4 |
[3H]CGP39,653, 50 nM | 362 ± 61 | 3 |
125I-Dynorphin A(2-17), 10 nM | 1456 ± 34 | 2 |
↵1-a Net specific binding of a ligand was determined by subtracting the specific binding of the ligand in nontransfected cells from that in transfected cells in parallel binding assays using the same amount of membrane protein per assay.