Table 1

Rate of PG and 6-MP accumulation in buffer, liver, and kidney homogenate

	Rate of Accumulation
	PG	6-MP
	nmol/ml/min	pmol/ml/min
Buffer	1.12 ± 0.02	5.4 ± 0.8
Liver homogenate	1.08 ± 0.04	10.6 ± 0.9^1-a
Kidney homogenate	0.30 ± 0.05^1-c	38.0 ± 3.4^1-b

PTA (1 mM), GSH (5 mM), and allopurinol (5 mM) were incubated in the presence of buffer, liver, or kidney homogenate. Protein concentration in liver and kidney homogenate incubations was 2.32 ± 0.03 and 2.50 ± 0.13 mg, respectively. Samples were taken every 15 min and analyzed by HPLC as described in Materials and Methods.Values presented are the mean ± S.D. from three independent experiments.

↵1-a Significantly different from buffer-only (p < .05).
↵1-b Significantly different from buffer-only and liver homogenate (p < .05).
↵1-c No increase in PG accumulation was observed after 15 min in kidney homogenate incubations.