Table 5

Effect of TAK-778 on cell number and ALP activity of C3H10T1/2 cells and rat calvarial cells

Cell NumberALP Activity
×105/well nmol/min/well
C3H10T1/2 cells
 Control11.5  ± 0.31.8  ± 0.6
 TAK-778
  10−7 M11.8  ± 0.10.5  ± 0.2
  10−6 M13.0  ± 0.35-150 0.2  ± 0.15-150
  10−5 M15.4  ± 0.1** 0.4  ± 0.25-150
Rat calvarial cells
 Control6.3  ± 0.250.6  ± 0.5
 TAK-778
  10−7 M7.1  ± 0.35-150 57.0  ± 0.8
  10−6 M6.9  ± 0.166.4  ± 0.7**
  10−5 M5.0  ± 0.2** 87.9  ± 9.6**

Cells were cultured in medium with or without various concentrations of TAK-778 for 7 days. Mean ± S.E. (n = 5).

  • ↵5-150 p < .05; **p < .01 versus control, Dunnett’s multiple comparison.