Table 1

Effect of 2.5 nM PD 115199 added at different times on intracellular cAMP accumulation induced by 1 and 10 μM forskolin over 60 min

Drug AdditioncAMP
At 0 minAt 60 minAt 60 minAt 120 min
pmol/106cells
Forskolin, 1 μM660  ± 54581  ± 54
Forskolin, 1 μM + 324  ± 30**
 PD 115199, 2.5 nM(−52%)
Forskolin, 1 μMPD 115199, 2.5 nM 306  ± 18**
 (−48%)
Forskolin, 10 μM2071  ± 2011319  ± 114
Forskolin, 10 μM + 1393  ± 118**
 PD 115199, 2.5 nM(−33%)
Forskolin, 10 μMPD 115199, 2.5 nM 858  ± 106*
(−35%)

  • Data are the means ± S.E.M. of six to eight replicates. Results were compared using an ANOVA followed by the Newman-Keuls range test. The asterisks (*P < .05; ** P< .01) denote significance of PD 115199 treatment at different times on forskolin activity.