Effects of temperature, osmolarity, endocytosis inhibitors, and metabolic inhibitor on the acid-resistant binding of125I-labeled C-AVP4-9 to MBEC4
| Condition | Acid-Resistant Binding |
|---|---|
| % of control | |
| Control | 100 ± 7.4 |
| Low temperature (4°C) | 10.4 ± 1.23-c |
| Hypertonic (1600 mOsM) | 53.4 ± 14.93-b |
| Dancylcadaverine (0.5 mM) | 74.3 ± 3.73-b |
| 2,4-Dinitrophenol (1 mM) | 26.0 ± 3.93-c |
| Control3-a | 100 ± 14.6 |
| Phenylarsine oxide (0.1 mM)3-a | 44.4 ± 8.03-b |
After preincubation for 30 min, 125I-labeled C-AVP4-9 and [3H]inulin were incubated under the indicated conditions at 4° or 37°C for 60 min. Each value represents the percentage of acid-resistant binding of125I-labeled C-AVP4-9 (as cell/medium ratios) versus the control value (mean ± S.E. of three or four separate experiments).
↵3-a Because phenylarsine oxide was dissolved in 0.1% dimethyl sulfoxide, the control experiment was performed in the presence of 0.1% dimethyl sulfoxide.
↵3-b Significant inhibition (p < .05).
↵3-c Significant inhibition (p < .001).