Effect of inhibitors of cyclooxygenase and 5-lipoxygenase on human eosinophil apoptosis
| Treatment | Apoptotic Eosinophils at 48 h (% total cells) |
|---|---|
| Control | 29.1 ± 4.6 (6) |
| Indomethacin (1 μM) | 27.7 ± 4.8 (6) |
| Flurbiprofen (1 μM) | 32.5 ± 5.3 (6) |
| L-745,337 (1 μM) | 28.9 ± 4.8 (6) |
| Control | 30.7 ± 1.1 (3) |
| Bay ×1005 (3 μM) | 32.7 ± 2.1 (3) |
| Bay ×1005 (30 μM) | 26.2 ± 2.1 (3) |
The percentage of apoptotic eosinophils after 48-h culture with inhibitors of cyclooxygenase and 5-lipoxygenase was determined by flow cytometric analysis of relative DNA content. Data represent the mean ± S.E.M. of n independent determinations from different cell preparations shown in parentheses.
Values in parentheses indicate number of determinations from different donors.