Table 4

Effect of DEP on ongoing CIA

DEP	Mean Joint Score	Anti-CII IgG (A405)	Anti-CII IgG2a (A405)	IFN-γ	IL-2	IL-4
mg/ml				nmol
0 (PBS)	3.4 ± 0.3	0.57 ± 0.06	0.40 ± 0.03	227 ± 13.3	254 ± 24.8	36 ± 2.1
0.1	3.9 ± 0.5	0.71 ± 0.06	0.44 ± 0.04	243 ± 22.3	263 ± 29.2	39 ± 3.2
0.3	5.6 ± 0.6^4-150	1.08 ± 0.09^4-150	0.67 ± 0.04^4-150	365 ± 20.9^4-150	343 ± 28.4^4-150	48 ± 4.1^4-150
1	6.5 ± 0.6^4-150	1.52 ± 0.12^4-150	0.83 ± 0.06^4-150	465 ± 36.5^4-150	566 ± 38.8^4-150	59 ± 4.9^4-150

To induce CIA, mice were immunized with CII on day 0 followed by a booster injection on day 21. Fifty microliters of PBS alone or 50 μl of PBS containing 0.1, 0.3, and 1 mg/ml DEP were intranasally administered daily from days 31 to 45. The severity of CIA, production of serum anti-CII IgG and IgG2a antibodies, and in vitro secretion of IFN-γ, IL-2, and IL-4 were determined on day 50 as described inMaterials and Methods. Values are the mean ± S.E.M. of eight mice or quadruplicate cultures of cells pooled from eight mice. Data are representative of two experiments.

↵4-150 p < .05 versus PBS alone, Mann-Whitney analysis.