Days after Immunization | DEP | |||
---|---|---|---|---|
0 (PBS) | 0.1 mg/ml | 0.3 mg/ml | 1 mg/ml | |
18 | 0 /12 (0)1-a | 0 /12 (0) | 0 /12 (0) | 0 /12 (0) |
21 | 0 /12 (0) | 0 /12 (0) | 1 /12 (8) | 3 /12 (25) |
24 | 0 /12 (0) | 0 /12 (0) | 3 /12 (25) | 5 /12 (42)1-150 |
27 | 2 /12 (17) | 3 /12 (25) | 5 /12 (42) | 8 /12 (67)1-150 |
30 | 4 /12 (33) | 6 /12 (50) | 6 /12 (50) | 10 /12 (83)1-150 |
35 | 5 /12 (42) | 7 /12 (58) | 8 /12 (67) | 12 /12 (100)1-150 |
40 | 5 /12 (42) | 7 /12 (58) | 9 /12 (75)1-150 | 12 /12 (100)1-150 |
To induce CIA, mice were immunized with CII on day 0 and boosted on day 21 as described in Materials and Methods. Fifty microliters of PBS alone or 50 μl of PBS containing 0.1, 0.3, and 1 mg/ml of DEP were intranasally administered every 2 days from days 0 to 20. The incidence of arthritis was examined on the indicated days.