Table 2

Effect of DEM concentration on hepatocyte concentrations and fractions unbound in media, cells and microsomes

Initial Concentration	Cell Concentration^2-a	Cell-to-Media Partition Coefficient	Fraction Unbound
Initial Concentration	Cell Concentration^2-a	Cell-to-Media Partition Coefficient	Media^2-b	Cells^2-c	Microsomes
μM	μM
0.5	18.3 ± 2.4	48.3 ± 7.2	0.85 ± 0.02	0.021 ± 0.003	1.00 ± 0.02
5	208 ± 17	53.3 ± 3.4	0.83 ± 0.01	0.019 ± 0.002	1.02 ± 0.02
50	1373 ± 85	33.5 ± 1.5	0.89 ± 0.01	0.030 ± 0.001	0.96 ± 0.06
500	7154 ± 1219	15.9 ± 3.1	0.94 ± 0.01	0.064 ± 0.011	0.96 ± 0.01