Table 1

Time dependence of serum-induced cell-cycle progression in synchronized A10 vascular smooth muscle cells

Time Since Serum RepletionG0/G1SG2/M
% % %
10% FCS 0 h86.7  ± 0.81-160 12.1  ± 1.41-160 1.1  ± 0.8
17 h57.0  ± 0.743.0  ± 0.72.3  ± 0.8
18 h41.0  ± 0.3155.0  ± 3.24.0  ± 1.5
19 h48.2  ± 1.338.2  ± 2.813.5  ± 4.2
20 h45.8  ± 2.629.4  ± 2.624.8  ± 0.1
10% FCS (18 h) + 10−8 Ml-citrulline45.1  ± 5.240.0  ± 1.21-150 7.7  ± 2.5

Times given are those transpiring between serum repletion and cell harvesting. Cell distribution was evaluated by flow cytometric determination of DNA content.

  • 1-150P < .05 compared with 10% FCS-treated cells during 18 h.

  • 1-160P < .01.