Michaelis-Menten parameters of quinidine metabolism by recombinant human CYP 3A4 in the presence of meloxicam
| Meloxicam | Km | Vmax | B | Vmax/Km |
|---|---|---|---|---|
| μM | μM | arbitrary units | arbitrary units/μM | |
| 0 | 84 | 9.5 | 0.9950 | 0.11 |
| 10 | 88 | 9.1 | 0.9990 | 0.10 |
| 100 | 56 | 6.6 | 0.9984 | 0.12 |
| 500 | 38 | 4.2 | 0.9986 | 0.11 |
| 750 | 38 | 3.7 | 0.9992 | 0.10 |
Michaelis-Menten parameters Km andVmax with and without quinidine were calculated from V/S plots by nonlinear regression analysis (mean of duplicate experiments).