Amount of plasmid DNA and liposomal lipid associated with lavage fluid, blood, and tumors at various time points after i.p. administration of free DNA, cationic DODAC/DOPE liposomes, and DODAC/DOPE liposome/DNA aggregates. Plasmid levels were determined using [3H]-labeled DNA and liposome levels were measured using [14C]-labeled DOPE as a lipid marker. Results are expressed per milliliter blood, per gram tumor tissue, and total lavage (3 ml) as mean ± S.E. for at least n = 5.
| Time | Free DNA | Liposomes | DODAC/DOPE DNA Lipoplexes | |
|---|---|---|---|---|
| DNA | Lipid | |||
| min | μg | nmol | μg | nmol |
| Blood | ||||
| 30 | 0.7 ± 0.1 | ND1-a | 0.3 ± 0.1 | ND |
| 60 | 0.9 ± 0.1 | ND | 0.6 ± 0.1 | ND |
| 120 | 0.9 ± 0.1 | ND | 0.7 ± 0.1 | ND |
| Lavage | ||||
| 30 | 7.60 ± 2.04 | 34.04 ± 14.86 | 2.50 ± 0.23 | 46.54 ± 5.72 |
| 60 | 6.70 ± 2.03 | 50.32 ± 5.33 | 3.43 ± 0.54 | 53.32 ± 8.72 |
| 120 | 5.41 ± 1.16 | 41.80 ± 9.33 | 3.98 ± 0.42 | 55.60 ± 7.76 |
| Tumor | ||||
| 30 | 4.74 ± 0.096 | 140.61 ± 57.98 | 21.21 ± 3.34 | 279.80 ± 46.01 |
| 60 | 6.60 ± 1.12 | 270.89 ± 45.95 | 22.14 ± 2.34 | 270.81 ± 33.11 |
| 120 | 7.85 ± 1.03 | 285.76 ± 45.96 | 46.01 ± 6.57 | 497.50 ± 60.11 |
↵1-a Not detectable.