Patient | DCE Metabolites | |
---|---|---|
DCE-Epoxide | 2,2-Dichloroacetaldehyde | |
pmol/mg protein/min | ||
HL-1 | 29.4 ± 3.0 | 5.8 ± 0.3 |
HL-2 | 19.3 ± 5.2 | 4.1 ± 0.5 |
HL-3 | 18.5 ± 4.6a,b | 4.7 ± 0.8 |
HL-4 | 18.5 ± 3.3a,b | 5.6 ± 0.4 |
HL-5 | 34.9 ± 12.8 | 6.3 ± 0.7 |
HL-6 | 28.1 ± 3.2 | 5.7 ± 1.3 |
HL-7 | 23.2 ± 2.9 | 4.5 ± 0.7 |
HL-8 | 15.6 ± 4.2a,b | 5.0 ± 0.7 |
Murine lung | 40.3 ± 3.81-c | 9.7 ± 2.0 |
Reaction mixtures in a total volume of 0.5 ml contained 5 mg/ml microsomal protein, [14C]DCE (2 mM, specific activity 7.5 nCi/nmol), and an NADPH-generating system; incubations were performed at 25°C for 30 min. Levels of DCE-epoxide were estimated from the total amount of conjugates [B] and [C], and 2,2-dichloroacetaldehyde was estimated from the amount of the acetal. Data from human lung represent the mean ± S.D. of triplicate determinations from microsomal preparations from individual patients. Data from murine lung represent the mean ± S.D. of triplicate determinations from three separate microsomal preparations.
1-a Significantly different from HL-1 (p< .05).
1-b Significantly different from HL-5 (p < .05).
↵1-c Significantly different from all human lung samples except HL-5.