Table 1

Physicochemical properties of CAT derivatives used in study

CompoundNumber of NH2-Group1-a% of Modified NH2-Group1-bApparent Molecular Weight1-cEnzymatic Activity1-d
CAT112.0235000100.0
Suc-CAT32.071.425000086.4
PEG-CAT85.623.683600096.2
Gal-CAT40.763.723500090.2
Man-CAT33.070.523500096.5

Results are expressed as mean of three determinations.

  • ↵1-a Determined by TNBS method.

  • ↵1-b Expressed as percentage of number of NH2-group of CAT.

  • ↵1-c Estimated by HPLC gel filtration chromatography.

  • ↵1-d Expressed as percentage of CAT.