Apparent kinetic constants for zidovudine, umbelliferone, oxazepam, lamotrigine, and glucuronidation and lidocaine oxidation by healthy and unhealthy liver microsomes
| Substrates | Healthy Livers | Cirrhosis | Fulminans Hepatitis (n = 2) | |||
|---|---|---|---|---|---|---|
| Vmax | Km | Vmax | Km | Vmax | Km | |
| nmol/min/mg | mM | nmol/min/mg | mM | nmol/min/mg | mM | |
| Lidocaine | 6.35 ± 2.88 | 2.97 ± 2.02 | 1.10 ± 1.402-a | 3.00 ± 4.10 | 0.10–0.08 | 0.37–1.24 |
| (n = 19) | (n = 19) | (n = 11) | (n = 11) | |||
| Zidovudine | 1.05 ± 0.46 | 3.72 ± 2.00 | 0.31 ± 0.162-a | 2.37 ± 2.36 | 0.07–0.04 | 1.16–4.5 |
| (n = 12) | (n = 12) | (n = 11) | (n = 11) | |||
| Umbelliferone | 32.4 ± 27.2 | 0.36 ± 0.26 | 21.5 ± 14.1 | 0.28 ± 0.20 | 4.5–2.9 | 0.27–0.08 |
| (n = 12) | (n = 12) | (n = 11) | (n = 11) | |||
| Oxazepam | 0.0034 ± 0.0009 | 0.30 ± 0.23 | 0.0028 ± 0.0001 | 0.19 ± 0.12 | 7 · 10−5 –12 · 10−5 | 0.045–0.053 |
| (n = 10) | (n = 10) | (n = 9) | (n = 9) | |||
| Lamotrigine | 0.96 ± 0.38 | 5.49 ± 5.15 | 0.85 ± 0.54 | 4.30 ± 1.98 | 0.03–0.05 | 1.17–6.6 |
| (n = 12) | (n = 12) | (n = 10) | (n = 10) | |||
↵2-a P < .01 versus healthy livers.