Interday variability of ER in modified Caco-2 cells
| Experiment No.3-a | Incubation time | Total 1′-OH-MDZ3-b | Basolateral MDZ | Basolateral MDZ | ER3-c |
|---|---|---|---|---|---|
| min | pmol | pmol | % of Apical Dose | ||
| 1 | 30 | 66.1 | 374 | 9.1 | 15.1 |
| 2 | 20 | 35.0 | 264 | 9.9 | 11.7 |
| 3 | 30 | 90.9 | 375 | 8.3 | 19.5 |
| 4 | 10 | 53.2 | 280 | 5.2 | 16.0 |
| 5 | 20 | 44.6 | 347 | 10.0 | 11.4 |
| 6 | 20 | 57.1 | 374 | 8.9 | 13.2 |
| Range | 11.4–19.5 | ||||
| Average | 14.5 | ||||
| SD | 3.1 | ||||
| CV | 21.1 |
↵3-a Experiment number refers to experiments where 4.5 nmol of MDZ was dosed apically and 10% or less of this dose crossed into the basolateral compartment after the indicated incubation time.
↵3-b Total 1′-OH-MDZ is the sum of the amount found in the apical and basolateral medium and the amount remaining in the cell at the end of the incubation time.
↵3-c The first-pass ER was calculated by application of the total amount of 1′-OH MDZ formed and total MDZ in the basolateral medium to eq. 2.