Time After Dose | Apical Dose A/B Ratio | Basolateral Dose A/B Ratio |
---|---|---|
min | ||
20 | 1.8 | 2.2 |
30 | 2.8 | 2.3 |
45 | 3.8 | 2.7 |
60 | 1.6 | 2.4 |
90 | 1.6 | 2.0 |
120 | 2.9 | 2.0 |
Mean | 2.4 | 2.3 |
S.D. | 0.90 | 0.27 |
Caco-2 monolayers were treated with 3 μM MDZ in either the apical or basolateral compartment. Total 1′-OH MDZ was measured in the apical and basolateral medium after incubating for the indicated period of time. Each ratio represents the result obtained from a single culture.