Table 1

Oligonucleotides used in this study

PrimerSequenceRestriction site to be generatedPosition1-a
2A6-F35′-CCACCATGCTGGCCTCAGGG-3′5–24
2A6-1AS-25′-TTCATGAGGGAGTTGTACATCTG-3′166–188
2A6-8S5′-CACCGAAGTGTACCCTATGCTG-3′1173–1194
2A7-B15′-CACCGAAGTGTTCCCTATGCTG-3′1173–1194
2A6-B65′-CGATGGAAAAGGGCACAAAAGCA-3′1290–1312
2A6-9S5′-GAAAGCGGAACTGTTTCGGAGAA-3′1313–1335
2A6-B15′-CAAGCTTAGCTGCGCCCCTCT-3′ Hind III1565–1585
  • 1-a Number corresponds to the location described previously by Yamano et al (1990). Recognition sites for restriction endonucleases used for subcloning of PCR fragments are underlined.