Adenylyl cyclase activity in sham-operated animals
| Region and Measure | Young | Aged |
|---|---|---|
| Temporal/Occipital Cortex | ||
| Basal (pmol/mg protein/min) | 68 ± 4 | 49 ± 3* |
| +GTP | 127 ± 8 | 103 ± 6* |
| +GTP+Fluoride | 180 ± 12 | 159 ± 9 |
| +GTP+Forskolin+Mn2+ | 6506 ± 665 | 7820 ± 512 |
| % Stimulation by Isoproterenol | 15 ± 2 | 19 ± 3 |
| Cerebellum | ||
| Basal (pmol/mg protein/min) | 97 ± 5 | 68 ± 4* |
| +GTP | 166 ± 9 | 144 ± 7 |
| +GTP+Fluoride | 249 ± 18 | 285 ± 18 |
| +GTP+Forskolin + Mn2+ | 4447 ± 403 | 7088 ± 514* |
| % Stimulation by Isoproterenol | 119 ± 15 | 142 ± 17 |
| Striatum | ||
| Basal (pmol/mg protein/min) | 42 ± 1 | 31 ± 1* |
| +GTP | 71 ± 4 | 62 ± 3* |
| +GTP+Fluoride | 135 ± 12 | 109 ± 9 |
| +GTP+Forskolin+Mn2+ | 5320 ± 507 | 6994 ± 361 |
| % Stimulation by Dopamine | 40 ± 9 | 47 ± 5 |
Data represent means and standard errors obtained from 10 animals in each group. Stimulation by isoproterenol or dopamine represents the percentage increase over those obtained with GTP alone. Across all three regions, ANOVA indicates significant age-related differences that depend on the type of cyclase measurement (age × measure interaction, p < .0001) and the interaction is also present (p < .0001) in each region taken individually. Asterisks denote individual measures that differ between young and aged cohorts.