Saturation binding results to untreated, control bovine striatal membranes or pretreated with CACO, N-CPM-CACO, CAM, or MC-CAM
| Ligands | Kd | Bmax |
|---|---|---|
| nM | fmol/mg of protein | |
| Control | 0.63 ± 0.05 | 130 ± 4 |
| 50 nM CACO | 0.52 ± 0.14 | 29 ± 9 |
| 50 nM N-CPM-CACO | 0.46 ± 0.06 | 25 ± 1 |
| 8 nM CAM | 1.1 ± 0.11 | 48 ± 3 |
| 64 nM MC-CAM | 2.4 ± 0.43 | 73 ± 7 |
Membranes were incubated with 50 nM CACO, 50 nM N-CPM-CACO, 8 nM CAM, or 64 nM MC-CAM for 15 min at 25°C. Control samples lacked compound in preincubation. Membranes were then diluted with 50 mM Tris-HCl, pH 7.5, followed by four centrifugal washes. Membranes were finally resuspended in 50 mM Tris-HCl, pH 7.5, and [3H]DAMGO binding at concentrations ranging from 0.025 to 3.2 nM was measured as described in Materials and Methods. Data are expressed as mean value ± S.E.M. for three determinations performed in triplicate.