Dissociation constants (Kd andKd, app), apparent exchangeable fractions (fapp), and unbound fraction in buffer containing 0.5 mM BSA
| Parameter | Diazepam | Caffeine | Propranolol | S-312-d | (+)-S-145 Na | S-8510 | |
|---|---|---|---|---|---|---|---|
| i.c.a. injection method | K d, app(mM) | 13.9 | 4.99 | 0.248 | 0.310 | 0.0358 | 223,121 |
| (11.3) | (1.19) | (0.025) | (0.052) | (0.0044) | |||
| f app(%) | 96.5 | 90.9 | 33.1 | 38.3 | 6.68 | 100 | |
| Perfusion method | K d, app(mM) | ND | 19,999 | ND | 0.170 | 0.0212 | ND |
| (0.060) | (0.0026) | ||||||
| f app(%) | ND | 100 | ND | 25.4 | 4.07 | ND | |
| Ultrafiltration in vitro | K d1(mM) | 0.00201 | 4.90 | ND | ND | 0.0346 | 0.980 |
| K d2(mM) | 0.270 | ND | ND | 1.25 | |||
| Unbound fraction (%) | 11.6 | 84.3 | ND | <12-a | 2.99 | 63.7 | |
ND, not determined. S.D. given in parentheses (detail for Method).
↵2-a Value for rat serum.