Cell Treatment | NS398 | PGE2 | Protein Level |
---|---|---|---|
% | arbitrary units | ||
None | − | 100.0 | 100 ± 8 |
+ | 9.8 | 533 ± 47 | |
Arachidonate, 50 μM | − | 135.1 | 128 ± 11 |
+ | 15.2 | 514 ± 44 | |
PGE2, 10 μM | − | N.D. | 103 ± 9 |
+ | N.D. | 501 ± 51 | |
PGJ2, 10 μM | − | 98.5 | 101 ± 10 |
+ | 7.3 | 498 ± 46 | |
15-deoxy-PGJ2, 10 μM | − | 80.2 | 75 ± 62-a |
+ | 6.1 | 382 ± 352-a |
Cells were stimulated for 24 h with LPS (1 μg/ml) and the indicated lipids in the absence or presence of NS398 (50 μM). The accumulation of PGE2 in the culture medium was measured as well as the amount of COX-2 protein (Western blot). Results show the mean of three experiments (± S.E.M. for the protein levels). 100% of PGE2 synthesis corresponded to 65 ng/mg protein. A value of 100 of COX-2 protein corresponded to the intensity of the band obtained in cells incubated with LPS.
N.D., not determined.
↵2-a P < .05 versus the None condition.