Table 1

The effect of native Pardaxin versus synthetic Pardaxin peptides on PGE2 release from PC12 cells1-a

PeptideSequence1-bPeptide-induced PGE2 release (pg/ml)
NativeGFFALIPKIISSPLFKTLLSAVGSALSSSGGQE750 ± 55
SyntheticGFFALIPKIISSPLFKTLLSAVGSALSSSGGQE660 ± 85
Analog [D]P7 GFFALIPKIISSPLFKTLLSAVGSALSSSGGQE-(NH2)2 ND1-c
Analog [D]L18L19 GFFALIPKIISSPLFKTLLSAVGSALSSSGGQE-(NH2)2 ND1-c
Analog [D]P7L18L19 GFFALIPKIISSPLFKTLLSAVGSALSSSGGQE-(NH2)2 ND1-c

  • ↵1-a PC12 cells were incubated with Pardaxin peptides (5 μM) for 15 min at 37°C, and PGE2 release was determined in the culture medium. The results are the mean ± S.E.M. of three experiments (n = 3). The basal release of PGE2(217 ± 82 pg/ml) was subtracted from all the experimental values.

  • ↵1-b Amino acids marked by bold letters were substituted with their d-enantiomers; E-(NH2)2 stands for glutamic acid in which the two COOH groups were modified to two CO-NH-CH2CH2-NH2 groups by transamination with ethylenediamine.

  • ↵1-c ND, nondetectable.