Compound | IC50Value | Cross-reactivity (%) |
---|---|---|
15-epi-LXA4 | 50 pg | 100 |
15-epi-LXA4 methyl ester | 60 pg | 83.0 |
LXA4 | 8 ng | 0.63 |
LXB4 | 40 ng | 0.13 |
15-R/S-methyl LXA4 | 20 ng | 0.25 |
15-epi-LXB4 methyl ester | 30 ng | 0.17 |
5S-HETE | 20 ng | 0.25 |
12S-HETE | >100 ng | <0.05 |
15S-HETE | 40 ng | 0.13 |
15R-HETE | 4 ng | 1.25 |
IC50 values represent the amount of the compound required to displace 50% of the maximum binding with the HRP-linked 15-epi-LXA4. The cross-reactivity of the antiserum to an individual compound was expressed as (IC50 value of 15-epi-LXA4/IC50 value of the compound) × 100%.