Effect of subacute oral administration of Sch 50971 and standard sedating drugs on pentobarbital-induced loss of righting reflex in guinea pigs
| Treatment3-a | Group | Loss of righting reflex3-b | Increase in sleep over group I |
|---|---|---|---|
| min ± S.E.M. | min | ||
| Vehicle 5 days | I | 89 ± 4 | — |
| Vehicle 4 days, then Sch 50971 (10 mg/kg) 1 dose | II | 116 ± 43-c | +26 |
| Sch 50971 (10 mg/kg) twice daily for 4 days, then one dose on day 5 | III | 118 ± 83-c | +29 |
| Vehicle 4 days, then diphenhydramine (30 mg/kg) 1 dose | IV | 159 ± 153-c | +63 |
| Diphenhydramine (30 mg/kg) twice daily for 4 days, then one dose on day 5 | V | 141 ± 163-c | +46 |
| Vehicle 4 days, then triazolam (1 mg/kg) 1 dose | VI | 213 ± 223-c | +118 |
| Triazolam (1 mg/kg) twice daily for 4 days, then one dose on day 5 | VII | 153 ± 15c,d | +57 |
↵3-a Animals were dosed orally twice each day (first dose 8:00 a.m., second dose 3:30 p.m.) for 4 days. On the fifth day, groups II and III received a single dose of Sch 50971 10 mg/kg p.o.; groups IV and V received a single dose of diphenhydramine 30 mg/kg, p.o.; groups VI and VII received a single dose of triazolam 1 mg/kg, p.o. Group I received oral methylcellulose vehicle twice a day, and once on the 5th day.
↵3-b Loss of righting reflex is defined as time when animal is unable to right itself three times in a 1-min period. Values are the mean ± S.E.M. (n = 5–6).
↵3-c Statistically different from group I (P < .05).
3-d Statistically different from group VI (P < .05).