Characterization of [3H]5HT evoked release from mouse uterine horns
| Experimental Conditions | [3H]5HT Evoked Release2-a | |
|---|---|---|
| Compound 48/80 10 μg/ml | High K+ 100 mM | |
| Mouse uterine horns | ||
| Estrus | 4.23 ± 0.12 (3) | 0.67 ± 0.26 (3)2-b |
| Diestrus | 3.93 ± 0.69 (3) | 0.69 ± 0.14 (4)2-b |
| 6OHDA mouse uterine horns | ||
| Estrus | 4.53 ± 0.39 (3) | n.d. (4)2-b |
| Diestrus | 4.35 ± 0.53 (3) | n.d. (4)2-b |
Values were calculated as the net fractional 3H outflow above basal levels from the application of the stimulus (see fig. 3), and were expressed as means ± S.E. for the number of experiments set in parentheses. Veratridine 20 nmol/ml evoked a 3H outflow of 0.47 ± 0.22 (n = 4) in mouse uterine horns preparations.
↵2-a Corresponds to the difference of % of fractional [3H] release above resting levels.
↵2-b P < .05 when compared to the effect of compound 48/80 in the uterine preparations incubated with RBS. n.d.: not detected,i.e., there was no net increase over basal levels.