Table 2

Characteristics of temocaprilat uptake into isolated hepatocytes (mean ± S.E., N = 3)

TreatmentUptake (% of Control)
Rotenone (30 μM)22.9  ± 2.6
FCCP (2 μM)28.7  ± 4.7
PCMBS (100 μM)15.7  ± 1.7
DIDS (100 μM)14.7  ± 3.2
4°C4.3  ± 0.0
Na+→Li+ 108.2  ± 1.5
  • The uptake of [3H]temocaprilat (0.1 μM) by the isolated hepatocytes was studied in the presence of metabolic inhibitor, sulfhydryl-modifying reagent and anion exchange inhibitor. These drugs were added to the cell suspension 5 min before the addition of [3H]temocaprilat. Effect of incubation temperature and Na+ was also examined.