Table 1

Kinetic parameters for the transport of temocaprilat and E217βG (mean ± S.E., N = 3)

Hepatocytesoatp1 COS-7
Vmax (nmol/min/mg)Km (μM)Pdif (μl/min/mg)CLuptake (μl/min/mg)Vmax (nmol/min/mg)Km (μM)CLuptake (μl/min/mg)
Temocaprilat0.21  ± 0.0320.9  ± 8.01.9  ± 0.314.0  ± 2.40.092  ± 0.02246.7  ± 15.92.3  ± 0.4
E217βG0.47  ± 0.126.5  ± 1.68.5  ± 4.581.3  ± 4.40.32  ± 0.1611.0  ± 3.926.2  ± 5.6
  • The experimental data shown in figures 2 and 5 were analyzed to determine the kinetic parameters.