Conditioned medium made in the presence of IL-10 evokes less epithelial dysfunction
| % of Control response | ||
|---|---|---|
| PBMC-SEB CM (50%) | PBMC-SEB CM (50%) made in the presence of IL-103-a | |
| Transepithelial resistance | 46.9 ± 3.2b | 71.2 ± 6.2c |
| ΔIsc to forskolin | 35.8 ± 3.8b | 71.1 ± 6.5c |
CM, conditioned media from PBMC (106/ml) exposed to SEB (1 μg/ml) for 24 hr.
↵3-a IL-10 (10 ng/ml) was added to PBMC+SEB cultures and CM collected 24 hr later. b c P < .05 compared with control and other group; n = 8 T84 monolayers; mean + S.E.M.