Culture conditions | |||
---|---|---|---|
Media only | T + B cells + SEB | T + B cells + SEB + IL-10 (10 ng/ml) | |
(1) T + B cells treated with IL-10 (10 ng/ml) for 24 hr before coculture | |||
Resistance (Ω/cm2) | 2887 ± 330 | 784 ± 236a | 1711 ± 290b |
ΔIsc to forskolin (μA/cm2) | 52.8 ± 3.0 | 24.3 ± 2.3a | 35.3 ± 1.8b |
(2) IL-10 (10 ng/ml) added 2 hr after the initiation of the coculture | |||
Resistance (Ω/cm2) | 1957 ± 385 | 639 ±120a | 776 ± 168a |
ΔIsc to forskolin (μA/cm2) | 67.7 ± 3.7 | 25.7 ± 0.4a | 31 ± 2.4a |
T + B cells, T cell-enriched, monocyte-depleted peripheral blood lymphocytes were used at 106 cells; forskolin was used at 10−5 M and SEB at 1 μg/ml.
a b P < .05 compared with medium control and to designate statistically different groups; n = 2–3 experiments with 2–4 T84 monolayers/condition; mean ± S.E.M.