Representative purification of the larger (250 kiloDalton) A-esterase from rat liver
| Fraction | Vol (ml) | Total Activity (μmol/Min) | Total Protein (mg) | Specific Activity2-a | Yield (%) |
|---|---|---|---|---|---|
| Whole homogenate | 123.4 | 701.26 | 7502.7 | 93.5 | |
| 1000 g supernate | 101.0 | 61.93 | 5765.1 | 107.4 | 88.3 |
| Microsomes | 80.0 | 395.58 | 1248.0 | 317.0 | 56.4 |
| Solubilized | 80.0 | 357.21 | 1280.0 | 279.1 | 51.0 |
| 100,000 g supernate | 76.0 | 332.21 | 874.0 | 380.1 | 47.4 |
| Ammonium sulfate precipitation | 6.2 | 31.30 | 78.8 | 397.2 | 4.0 |
| Gel filtration | 9.2 | 14.82 | 36.8 | 402.8 | 2.1 |
| PSIEF2-b(wells 4–6) ampholyte pH 5–8 | 3.1 | 0.62 | 3.4 | 179.4 | 0.09 |