Total Intensity (%) | Cell Coverage (%) | Average Intensity | |
---|---|---|---|
Red (65–128) | 41.2 | 54.9 | 96.7 ± 17.6 |
Green (129–191) | 43.2 | 35.6 | 156.5 ± 17.5 |
Blue (192–255) | 15.5 | 9.4 | 212.4 ± 16.8 |
Red represents the low level (diffuse) staining. Green and blue represent the mid-range and clustered staining, respectively, numbers in parentheses show the actual range of pixel intensities analyzed in each range. Values between 0 and 64 are considered as background noise in this example.