Affinities of selected PGs competing for specific [3H]PGF2α binding to BCLM membranes
| Compound | FP Receptor Affinity (Ki, nM) |
|---|---|
| 16-Phenoxy-PGF2α | 22 ± 5 (6) |
| Cloprostenol | 31 ± 3 (9) |
| 17-Phenyl-PGF2α | 59 ± 8 (5) |
| PHXA85 | 98 ± 11 (8) |
| Fluprostenol | 100 ± 12 (5) |
| PGF2α | 119 ± 9 (25) |
| Enprostil | 1,000 ± 230 (3) |
| 17-Phenyl-PGE2 | 1,413 ± 549 (4) |
| PGD2 | 2,500 ± 760 (3) |
| Sulprostone | 2,500 ± 350 (3) |
| PGE2 | 3,400 ± 710 (3) |
| Latanoprost (PHXA41) | 4,200 ± 790 (12) |
| UF-021 | 5,900 ± 710 (4) |
| U46619 | 8,900 ± 140 (3) |
| S-1033 | 22,000 ± 2,600 (5) |
| GR63799X | 30,000 ± 3,000 (3) |
| PGE1 | 40,000 ± 11,000 (5) |
| ZK118182 | 69,000 ± 19,000 (4) |
| PGI2 | 86,000 ± 29,000 (4) |
| BWA868C | 86,000 ± 12,000 (4) |
| 8-Iso-PGF2α | 120,000 ± 26,000 (8) |
Data are mean ± S.E.M. from 3 to 25 independent experiments as shown in parentheses. Note: all PGs listed, apart from GR63799X and latanoprost (which are esters), are the free acid forms of the compounds. For reference, the IC50/Ki values for PGF2α competing for [3H]PGF2α binding to homogenates/membranes of corpora lutea from different species have ranged from 30 to 50 nM (Powell et al., 1975; Rao, 1976; Lin and Rao, 1977; Goh and Kishino, 1994).