Effects of amphetamine on TH and GAPDH mRNA in midbrain as determined by RT-PCR
| Treatment | Time after amphetamine | TH PCR product | GAPDH PCR product | TH/GAPDH |
|---|---|---|---|---|
| days | pmol/10 μl of reaction mixture | |||
| Saline | 1 (5) | 98 ± 17 | 139 ± 16 | 0.70 ± 0.1 |
| Amphetamine | 1 (5) | 75 ± 17 | 97 ± 16 | 0.78 ± 0.1 |
| Saline | 3 (7) | 98 ± 15 | 149 ± 13 | 0.65 ± 0.09 |
| Amphetamine | 3 (8) | 85 ± 14 | 110 ± 13 | 0.87 ± 0.08 |
| Saline | 14 (7) | 96 ± 15 | 164 ± 13 | 0.55 ± 0.09 |
| Amphetamine | 14 (8) | 71 ± 14 | 140 ± 13 | 0.50 ± 0.08 |
| months | ||||
| Saline | 4 (7) | 91 ± 15 | 120 ± 13 | 0.78 ± 0.09 |
| Amphetamine | 4 (8) | 90 ± 14 | 131 ± 13 | 0.67 ± 0.08 |
Rats were treated with saline or amphetamine, and midbrain RNA was isolated at the time points. TH mRNA and GAPDH mRNA were measured using RT-PCR. The TH PCR product was produced using 21 cycles, whereas the GAPDH PCR product was produced using 19 cycles.
Data represent the mean ± S.E. from the number of rats designated in the parentheses.