Analysis of saturation studies of radiolabeled endomorphin
| 125I-Endomorphin-1 | 125I-Endomorphin-2 | |||
|---|---|---|---|---|
| KD (nM) | Bmax (fmol/mg protein) | KD (nM) | Bmax (fmol/mg protein) | |
| Mouse brain | 0.25 ± 0.06 | 7 ± 2 | 0.25 ± 0.1 | 6 ± 2 |
| MOR-1/CHO | 1.6 ± 0.5 | 311 ± 28 | 0.5 ± 0.1 | 148 ± 47 |
KD values ± S.E.M. were determined from saturation studies using nonlinear regression analysis, which was consistent with a single site for both radioligands in both tissue sources. Results are the means ± S.E.M. of at least three independent determinations.